荧光染料标记与染色：光物理性质数据库 Fluorescent Dye Labels And Stains - A Database Of Photophysical Properties

基本信息

Format:Hardback 480 pages

Publisher:John Wiley & Sons Inc

Imprint:John Wiley & Sons Inc

ISBN:9781119835134

Published:6 Apr 2023

Weight:1276g

Dimensions:244 x 170 (mm)

页面参数仅供参考，具体以实物为准

书籍简介

荧光染料标签和染色剂

全面的荧光团及其物理和光化学性质数据库

荧光团是强烈吸收紫外线、可见光和/或近红外线并在这些范围内发出明亮光的化合物。因此，它们作为各种分析过程的染料非常有价值，能够标记和染色特定目标以进行荧光成像、灵敏检测和定量（在非常宽的浓度范围内表现出线性响应）。这些化合物种类繁多，全面了解它们的选择、物理性质及其对光激发的反应对于成功整合到化学分析、药物分析、临床分析、显微镜、光学生物成像、癌症成像、实时 PCR、流式细胞术、蛋白质组学中的多路复用、一般生命科学以及许多其他高科技领域（材料科学、可追溯性、光伏、量子计算）至关重要。

荧光染料标签和染色剂包含此类物质及其特性的综合数据库。它介绍了基本理论和基础术语，以及大量荧光团的分子结构和光物理特性。该数据库汇集了一位杰出的生物化学家的宝贵经验，提供了以前从未在一卷书中提供过的宝贵信息。

读者还将发现：

·超过 700 种荧光团的分子和光化学信息

·参数数据库，包括光激发范围、摩尔吸收系数、荧光量子产率、分子亮度等

·来自多个学科的信息，包括显微镜、纳米显微镜、生物化学和分子生物学

·荧光染料标签和染色剂是制药和生物医学研究人员和专业人士、研究分子生物学或有机化学的学者以及任何工作涉及强光和光稳定的分子吸收和荧光的专业人士的基本参考资料。

Fluorescent Dye Labels and Stains

The only comprehensive database of fluorophores and their physical and photochemical properties

Fluorophores are chemical compounds that strongly absorb in the ultraviolet, visible, and/or near-infrared and with bright emission in these ranges. As a result, they are exceptionally valuable as dyes for various analytical processes, capable of labelling and staining particular targets for purposes of fluorescent imaging, sensitive detection, and quantification (exhibiting linear responses over very wide concentration ranges). These compounds are many and varied, and panoramic views of their options, physical properties and their reactions to light excitations can be critical to their successful integration into chemical analysis, pharmaceutical analysis, clinical analysis, microscopies, optical bioimaging, cancer imaging, real-time PCR, flow cytometry, multiplexing in proteomics, life sciences in general, and many other high-tech fields (material sciences, traceability, photovoltaics, quantum computing).

Fluorescent Dye Labels and Stains incorporates a comprehensive database of such substances and their characteristics. It provides an introduction to basic theories and foundational terminology, in addition to both the molecular structures and photophysical properties of an enormous range of fluorophores. Assembled over the course of a distinguished career in biochemistry, this database presents valuable information that has never before been available in a single volume.

Readers will also find:

Molecular and photochemical information of over 700 fluorophores

A database of parameters, including light excitation ranges, molar absorption coefficients, fluorescence quantum yields, molecular brightness, and many more

Information derived from multiple disciplines, including microscopy, nanoscopy, biochemistry, and molecular biology

Fluorescent Dye Labels and Stains is the essential reference for pharmaceutical and biomedical researchers and professionals, academics who study molecular biology or organic chemistry, and any professional whose work includes strong and photostable molecular absorptions and fluorescence.

作者简介

Tarso B. Ledur Kist 博士是巴西南里奥格兰德联邦大学生物物理系的全职教授。他发表了大量关于分子生理化学现象的理论建模、生命科学和食品分析中的荧光标记以及相关主题的论文。

Tarso B. Ledur Kist, PhD, is a Full Professor at the Department of Biophysics, Federal University of Rio Grande do Sul, Brazil. He has published widely on theoretical modeling of molecular physiochemical phenomena, fluorescent labelling in life sciences and food analysis, and related subjects.

部分目录，仅供参考

Preface x

Acronyms xii

Symbols and Conventionsxiii

1 Introduction 1

2 Basic Definitions and Fundamentals 5

2.1 Introduction 5

2.2 Light Sources 5

2.3 Filtering and Dispersing Light 6

2.3.1 Absorber Filters 6

2.3.2 Interference Filters 8

2.3.3 Polarizers 8

2.3.4 Prisms 9

2.3.5 Grating 9

2.4 Light Detectors 10

2.5 Light Beams 12

2.5.1 Radiant Power and Radiance in Space: Divergent and Collimated Beams 12

2.5.2 Radiant Power and Radiance in Time: Continuous, Modulated, and Pulsed 13

2.5.3 Spectral Radiant Power (Emission Spectra) of Lamps, LEDs, and Lasers 14

2.5.4 Light Wavelength, Transmittance, and Absorbance 14

2.5.5 Spontaneous Decay and Stimulated Emission in Lasers and STED Nanoscopy 16

2.5.6 Energy, Momentum, Polarization, Spin, and Angular Momentum 17

2.6 Light Collection Set-Ups 17

2.6.1 Microscope Objectives 17

2.6.2 Fluorescence Detection Set-Ups 18

2.6.3 Fluorescence Imaging Set-Ups 18

2.7 Fundamentals of Fluorescence 21

2.7.1 Fluorescence: Fields of Application 22

2.7.2 Molar Absorption Coefficient 23

2.7.3 Excitation Spectra 24

2.7.4 Emission Spectra 25

2.7.5 Stokes Shift 25

2.7.6 Fluorescence Quantum Yield 27

2.7.7 Brightness 27

2.7.8 Effective Brightness 27

2.7.9 Fluorescence Mean-Lifetime 28

2.7.10 Factors Affecting Fluorescence 29

2.7.10.1 Effect of Microenvironment 29

2.7.10.2 Influence of Liquid Viscosity on Fluorescence Quantum Yield and Fluorescence Mean-Lifetime 30

2.7.10.3 Influence of Electric Permittivity and Hydrogen Bonding 30

2.7.10.4 Effects of Temperature 31

2.7.10.5 Quenching 31

2.7.10.6 Self-Quenching 32

2.7.10.7 Singlet Oxygen Production by Sensitizer Dyes 32

2.8 Photostability 32

3 Target-Fluorophore Binding 37

3.1 Introduction 37

3.2 Choosing the Right Solvent 37

3.2.1 Water and PBS 37

3.2.2 Water Miscible Organic Solvents 39

3.3 Fluorogenic Reactions 45