Study on the Novelnear-infrared Fluorescent Probes Based on Indole Heptamethine Cyanine Dyes
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内容简介
近红外荧光成像技术作为一种非侵入性诊断技术，具有无辐射、无损、实时、在位检测生物信息等优点。菁染料作为近红外荧光染料中的一类，常被用作荧光检测标记物或制成荧光成像探针。吲哚七甲川菁染料是菁染料中应用多的一类，由吲哚杂环、七甲川链和N-取代基侧链组成，由于其具有避开机体组织自发荧光干扰和自吸收的能力，当前常被用作荧光成像探针，并成为研究的热点。而如何提高吲哚七甲川菁染料的光谱性能，光稳定性以及将其应用到生物活性检测当中则成为当前的研究重点。《新型吲哚七甲川菁类近红外荧光探针的设计合成及生物活性研究（英文版）》以吲哚七甲川菁染料为母核，设计、合成了一系列新的荧光探针，其分别具有较好的光稳定性、荧光成像性和生物检测活性等性能，期望本研究的一系列近红外荧光探针能够在荧光成像领域中得到广泛应用。
目录
1 Advances in the Biological Application of Indoles Heptamethine Cyanine Dyes Near-infrared Fluorescent Probes
1．1 Introduction
1．2 pH Application of Sensitive Near-infrared Fluorescent Probe
1．3 Application of Metal lon Selective Near-infrared Fluorescence Probe
1．4 Application of Small Molecule Sensitive Near-infrared Fluorescence Probe
1．5 Application of Labeled Tumor Cell Type Near-infrared Fluorescence Probe
1．6 Application of Near-intfrared Fluorescent Probe in Other Fields
1．7 Summary and Outlook
References
2 The Synthesis of a Near-infrared Fluorescent Probe IR-789 and Its Application in Imaging of MCF-7 Cells
2．1 Abstract
2．2 Introduction
2．3 Materials and Methods
2．3．1 Apparatus and Materials
2．3．2 Synthesis and Characterization of IR
2．3．3 Absorption Analysis
2．3．4 Fluorescence Analysis
2．3．5 MTT Assay
2．3．6 Confocal Imaging
2．4 Results and Discussion
2．4．1 Absorption and Fluorescence Spectra of IR
2．4．2 Test for Probe Selectivity
2．4．3 MTT Assay
2．4．4 Determination of Quantum Yield
2．4．5 Fluorescent Confocal Microscopy Image
2．5 Conclusion
References
3 A Novel Near-infrared Fluorescent Probe IR-736 was Synthesized and its Application in Imaging of Living Cells
3．1 Abstract
3．2 Introduction
3．3 Materials and Methods
3．3．1 Apparatus and Materials
3．3．2 Synthesis and Characterization of IR
3．3．3 Absorption Analysis
3．3．4 Fluorescence Analysis
3．3．5 MTT Assay
3．3．6 Confocal Imaging
3．4 Results and Discussion
3．4．1 The Absorption and Fluorescence Spectra of IR
3．4．2 MTT Assay
3．4．3 Determination of Quantum Yield
3．4．4 Fluorescent Confocal Microscopy Image
3．5 Conclusion
References
4 Synthesis of a Novel Near-infrared Fluorescent Probe IR-780 and Its Application in Imaging of Living Cells
4．1 Abstract
4．2 Introduction
4．3 Materials and Methods
4．3．1 Apparatus and Materials
4．3．2 Synthesis and Characterization of IR
4．3．3 Absorption Analysis
4．3．4 Fluorescence Analysis
4．3．5 MTT Assay
4．3．6 Confocal Imaging
4．4 Results and Discussion
4．4．1 Absorption and Fluorescence Spectra of IR
4．4．2 MTT Assay
4．4．3 Fluorescent Confocal Microscopy Image
4．5 Summary
References
5 Synthesis of a Novel IR-822-Met Near-infrared Probe for in Vivo Tumor Diagnosis
5．1 Abstract
5．2 Introduction
5．3 Materials and Methods
5．3．1 Apparatus and Materials
5．3．2 Synthesis and Characterization of IR-822-Met
5．3．3 Absorption Analysis
5．3．4 Fluorescence Analysis
5．3．5 MTT Assay
5．3．6 In Vitro Targeting Capability of IR-822-Met
5．3．7 Tumor Animal Models
5．3．8 In Vivo Targeting Capability of IR-822-Met
5．4 Results
5．4．1 The Absorption and Fluorescence Spectra of IR-822-Met
5．4．2 MTT Assay
5．4．3 Determination of Quantum Yield
5．4．4 In Vitro Targeting Capability of IR-822-Met
5．4．5 In Vivo Targeting Capability of IR-822-Met
5．4．6 Pathological Study of IR-822-Met
5．5 Conclusions
References
6 Synthesis of a Near-infrared Fluorescent Probe IR-822-DG for Tumor Diagnosis
6．1 Abstract
6．2 Introduction
6．3 Materials and Methods
6．3．1 Apparatus and Materials
6．3．2 Synthesis and Characterization of IR-822-DG
6．3．3 Absorption Analysis
6．3．4 Fluorescence Analysis
6．3．5 MTT Assay
6．3．6 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．3．7 Tumor Animal Models
6．3．8 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4 Results
6．4．1 Optical Properties of IR-822-DG
6．4．2 MTT Assay
6．4．3 Determination of Quantum Yield
6．4．4 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．4．5 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4．6 The Efect of the Probe on Tumor Growth and Animal Survival
6．5 Discussion
References
7 A Novel Near-infrared Probe for Imaging of O2 Fluctuations and HtEnhancement in HepG2 Cells
7．1 Abstract
7．2 Introduction
7．3 Experimental
7．3．1 Apparatus and Materials
7．3．2 Design and Sythesis of the Probe IR-747-SAPH
7．3．3 3-（4，5-Dimethythiazo1-2-y1）-2，5-diphenyl Tetrazolium Bromide（MTT） Assay
7．3．4 Cell Culture
7．3．5 Confocal Imaging
7．4 Results and Discussion
7．4．1 Photophysical Properties of IR-747-SAPH
7．4．2 Determination of Ouantum Yield
7．4．3 Selectivity of IR-747-SAPH
7．4．4 MTT Assay
7．4．5 Fluorescence Response Time and Reversibility of IR-747-SAPH
7．4．6 Confocal Fluorescence Images of Living Cells
7．5 Discussion
References
1．1 Introduction
1．2 pH Application of Sensitive Near-infrared Fluorescent Probe
1．3 Application of Metal lon Selective Near-infrared Fluorescence Probe
1．4 Application of Small Molecule Sensitive Near-infrared Fluorescence Probe
1．5 Application of Labeled Tumor Cell Type Near-infrared Fluorescence Probe
1．6 Application of Near-intfrared Fluorescent Probe in Other Fields
1．7 Summary and Outlook
References
2 The Synthesis of a Near-infrared Fluorescent Probe IR-789 and Its Application in Imaging of MCF-7 Cells
2．1 Abstract
2．2 Introduction
2．3 Materials and Methods
2．3．1 Apparatus and Materials
2．3．2 Synthesis and Characterization of IR
2．3．3 Absorption Analysis
2．3．4 Fluorescence Analysis
2．3．5 MTT Assay
2．3．6 Confocal Imaging
2．4 Results and Discussion
2．4．1 Absorption and Fluorescence Spectra of IR
2．4．2 Test for Probe Selectivity
2．4．3 MTT Assay
2．4．4 Determination of Quantum Yield
2．4．5 Fluorescent Confocal Microscopy Image
2．5 Conclusion
References
3 A Novel Near-infrared Fluorescent Probe IR-736 was Synthesized and its Application in Imaging of Living Cells
3．1 Abstract
3．2 Introduction
3．3 Materials and Methods
3．3．1 Apparatus and Materials
3．3．2 Synthesis and Characterization of IR
3．3．3 Absorption Analysis
3．3．4 Fluorescence Analysis
3．3．5 MTT Assay
3．3．6 Confocal Imaging
3．4 Results and Discussion
3．4．1 The Absorption and Fluorescence Spectra of IR
3．4．2 MTT Assay
3．4．3 Determination of Quantum Yield
3．4．4 Fluorescent Confocal Microscopy Image
3．5 Conclusion
References
4 Synthesis of a Novel Near-infrared Fluorescent Probe IR-780 and Its Application in Imaging of Living Cells
4．1 Abstract
4．2 Introduction
4．3 Materials and Methods
4．3．1 Apparatus and Materials
4．3．2 Synthesis and Characterization of IR
4．3．3 Absorption Analysis
4．3．4 Fluorescence Analysis
4．3．5 MTT Assay
4．3．6 Confocal Imaging
4．4 Results and Discussion
4．4．1 Absorption and Fluorescence Spectra of IR
4．4．2 MTT Assay
4．4．3 Fluorescent Confocal Microscopy Image
4．5 Summary
References
5 Synthesis of a Novel IR-822-Met Near-infrared Probe for in Vivo Tumor Diagnosis
5．1 Abstract
5．2 Introduction
5．3 Materials and Methods
5．3．1 Apparatus and Materials
5．3．2 Synthesis and Characterization of IR-822-Met
5．3．3 Absorption Analysis
5．3．4 Fluorescence Analysis
5．3．5 MTT Assay
5．3．6 In Vitro Targeting Capability of IR-822-Met
5．3．7 Tumor Animal Models
5．3．8 In Vivo Targeting Capability of IR-822-Met
5．4 Results
5．4．1 The Absorption and Fluorescence Spectra of IR-822-Met
5．4．2 MTT Assay
5．4．3 Determination of Quantum Yield
5．4．4 In Vitro Targeting Capability of IR-822-Met
5．4．5 In Vivo Targeting Capability of IR-822-Met
5．4．6 Pathological Study of IR-822-Met
5．5 Conclusions
References
6 Synthesis of a Near-infrared Fluorescent Probe IR-822-DG for Tumor Diagnosis
6．1 Abstract
6．2 Introduction
6．3 Materials and Methods
6．3．1 Apparatus and Materials
6．3．2 Synthesis and Characterization of IR-822-DG
6．3．3 Absorption Analysis
6．3．4 Fluorescence Analysis
6．3．5 MTT Assay
6．3．6 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．3．7 Tumor Animal Models
6．3．8 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4 Results
6．4．1 Optical Properties of IR-822-DG
6．4．2 MTT Assay
6．4．3 Determination of Quantum Yield
6．4．4 Targeting Ability of IR-822-DG in Vitro Tumor Cells
6．4．5 Targeting Ability of IR-822-DG in Tumor-Bearing Mice
6．4．6 The Efect of the Probe on Tumor Growth and Animal Survival
6．5 Discussion
References
7 A Novel Near-infrared Probe for Imaging of O2 Fluctuations and HtEnhancement in HepG2 Cells
7．1 Abstract
7．2 Introduction
7．3 Experimental
7．3．1 Apparatus and Materials
7．3．2 Design and Sythesis of the Probe IR-747-SAPH
7．3．3 3-（4，5-Dimethythiazo1-2-y1）-2，5-diphenyl Tetrazolium Bromide（MTT） Assay
7．3．4 Cell Culture
7．3．5 Confocal Imaging
7．4 Results and Discussion
7．4．1 Photophysical Properties of IR-747-SAPH
7．4．2 Determination of Ouantum Yield
7．4．3 Selectivity of IR-747-SAPH
7．4．4 MTT Assay
7．4．5 Fluorescence Response Time and Reversibility of IR-747-SAPH
7．4．6 Confocal Fluorescence Images of Living Cells
7．5 Discussion
References
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