生物技术演化方法：定向演化的灵巧技艺 Evolutionary Methods In Biotechnology - Clever Tricks For Directed Evolution

基本信息

Edited by:Susanne Brakmann, Andreas Schwienhorst

Format:Multiple-component retail product, part(s) enclose 227 pages

Publisher:Wiley-VCH Verlag GmbH

Imprint:Blackwell Verlag GmbH

ISBN:9783527307999

Published:22 Jun 2004

Weight:567g

Dimensions:246 x 175 (mm)

页面参数仅供参考，具体以实物为准

书籍简介

微型化和高通量分析技术将分子进化的力量带入了生物科学实验室。如果应用得当，进化方法可以快速产生预期结果，即使在其他方法失败的情况下也是如此。

从通过随机或定向诱变生成文库到筛选和选择技术——本实用指南详细描述了成功进化生物技术的关键步骤，其中还包括对常见问题的宝贵故障排除提示。

解释了肽和蛋白质表面展示的现代方法、核酸适体的选择性富集和工业生物催化剂的高通量筛选，并描述了基于计算机的计算机蛋白质和 RNA 工程方法作为体外方法的替代方案。一个特别部分涵盖了与定向进化衍生的生物技术创新有关的专利法规。

作为附加资源，还附有一张 CD-ROM，其中包含用于文库设计、诱变位置选择和各种预测算法的软件工具。

简而言之，这本以实践为导向的手册是每个从事这一跨学科研究领域的科学家不可或缺的工具。

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.

From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.

Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.

As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.

In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

作者简介

Susanne Brakmann 是莱比锡大学（德国）“应用分子进化”初级研究小组的负责人，也是莱比锡生物技术-生物医学中心的成员。她在布伦瑞克工业大学学习化学，并于 1988 年获得学位，之后前往卡尔斯鲁厄大学，在 Reinhold Tacke（1991 年获得博士学位）的指导下完成论文。她是哥廷根马克斯普朗克生物物理化学研究所的博士后研究员，在那里她与 Manfred Eigen 一起工作，之后于 2001 年搬到莱比锡。她对定向进化作为理解和优化酶功能的工具感兴趣，专注于核酸聚合酶及其生物技术应用。

Andreas Schwienhorst 是哥廷根大学（德国）微生物学和遗传学研究所的组长。他出生于瓦伦多夫，在明斯特和哥廷根攻读生物化学和史前史。他的论文是在马克斯普朗克生物物理化学研究所在曼弗雷德·艾根的指导下完成的。他曾在索尔克研究所、拉霍亚和洛斯阿拉莫斯国家实验室（美国）担任客座科学家，然后在分子生物技术研究所担任小组组长，之后于 1997 年转入哥廷根大学。1998 年，他获得了生物未来奖。目前，他的兴趣是发现药物干预的新靶点以及分子进化方法及其在药物发现和催化中的应用。

Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received herin 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Gottingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications.

Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Gottingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Munster and Gottingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Gottingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.